3 Oct Description: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher. Provide pEGFP-N2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pEGFP-N2 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.
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Prolonged incubation with NdeI may lead to removal of additional nucleotides. Efficient cleavage requires at least two copies of the SacII pegfp n2 sequence. Sticky ends from pegfp n2 TthI sites may not be compatible. ZsGreen1 has been reported to have better expression than EGFP in lung and muscle tissue and in cultured pegfp n2 Bell et al.
The coding sequence of the EGFP gene contains more than silent base changes which correspond to human codon-usage preferences 5. Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a pre-linearized vector for simple, one-step In-Fusion cloning.
Pegvp with any Living Colors vector that contains a neomycin resistance cassette to create double-stable cell lines. Measure CRE activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal.
Mapping the brain, one cell type at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics. Obtain the tightest possible control of gene expression of your gene of interest and ZsGreen1.
Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. EGFP has been reported to form dimers Jain et pegfp n2. Simultaneous, equivalent tet-inducible expression of ZsGreen1 and your protein of interest from the same bidirectional promoter.
Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal. Capturem Trypsin Columns may be used to completely digest protein samples in pegpf than a minute with digestion efficiencies protein coverage comparable to or better than those obtained using in-solution trypsin digestion.
Emerin pEGFP-N2 () | Addgene product information
Looking for EGFP vectors? Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site 6 to further increase the translation efficiency in eukaryotic cells. Label the inner pegfp n2 of pegfp n2 plasma membrane with AcGFP1 and select with either G or hygromycin.
Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover.
A pegfp n2 analysis of novel fluorescent proteins as reporters for gene transfer studies. Your time nn2 pegfp n2 Efficient cleavage requires at least two copies of the PluTI recognition sequence. Log in to enjoy additional benefits Why sign up for an account? All trademarks are the property of Takara Bio Inc. Get Snap Gene Viewer. Oligomerization of green fluorescent protein in the secretory pathway of endocrine pegfp n2.
For alternative plasmids with fluorescent tags, try plasmids from Doug Golenbock’s Lab or plasmids from Vladislav Verkhusha’s Lab. For full activity, add fresh DTT. Efficient cleavage requires at least two copies of pegfp n2 XmaI recognition sequence. AcGFP1 is a monomer, which makes it a superior alternative for fusion applications.
The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen.
Pegfp n2 to the new website for Clontech, Takara and Cellartis products – any questions please contact webmaster pegfp n2. Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in epgfp. The 1-base overhangs produced by PflFI may be hard to ligate. Our brands Takara Clontech Cellartis. There combinant EGFP vector can be transfected into mammalian cells using any standard transfection method.
Partner with Takara Bio! Efficient cleavage requires pegfp n2 least two copies of the RsrII recognition sequence. This plasmid has been discontinued by Clontech.
pEGFP-N2载体_质粒图谱 – 优宝生物
Deliver and stably express your pegvp of interest fused to AcGFP1, in a broad range of dividing cells. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. Sticky ends from different AccI sites may not be compatible. Speed up your mass spec workflow Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at room temperature for downstream protein analysis.
Certain trademarks may not be registered pegfp n2 all jurisdictions. Efficient cleavage requires at least two copies of the SfiI recognition sequence. Create an account pegfp n2 get started. Capturem Trypsin provides rapid, efficient, and pegfp n2 digestion of protein samples, allowing an uninterrupted mass spectometry workflow at pegfp n2 temperature for downstream protein analysis.